pegLIT

Automatically identify non-interfering nucleotide linkers between a pegRNA and 3' motif.

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SpacerScaffoldTemplatePBSLinker PatternMotif

GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC

8 bp

NNNNNNNN

tevopreQ1

CGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAA